Specific cleavage of kinetoplast minicircle DNA from Leishmania tarentolae by mung bean nuclease and identification of several additional minicircle sequence classes

Specific Deavage of Idnetoplast Minidrde Dna from Leishmania Tarentolae by Mung Bean Nudease and Identification of Several Additional Minidrde Sequence Classes

Multiple sequence classes of kinetoplast miniclrcle DNA from Le^iihmar^ia tarentolae were cleaved by mung bean nuclease In the presence of formamide, yielding unit length linear molecules which retained the anomalous electrophoretic mobility in acrylamide characteristic of minicircle DNA. No specific cleavage site sequence common to all minicircle sequence classes was apparent, although the mai...

Sequence heterogeneity and anomalous electrophoretic mobility of kinetoplast minicircle DNA from Leishmania tarentolae.

Several unit-length minicircles from the kinetoplast DNA of Leishmania tarentolae were cloned into pBR322 and into M13 phage vectors. The complete nucleotide sequences of three different partially homologous minicircles were obtained. The molecules contained a region of approx. 80% sequence homology extending for 160-270 bp and a region unique to each minicircle . A 14-mer was found to be conse...

Sequence heterogeneity in kinetoplast minicircle DNA of Leishmania majer

Structure of Leishmania Minicircle Kinetoplast DNA Classes

In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...

Structure of Leishmania Minicircle Kinetoplast DNA Classes

In a recent paper (5), Noyes et al. discussed a nested-PCRbased approach for identification and analysis of Leishmania kinetoplast DNA (kDNA) minicircle classes using the analysis of minicircle variable-region (VR) restriction profiles. The kDNA of trypanosomatids consists of catenated minicircle (approximately 5 3 10 to 5 3 10 per associate) and maxicircle (20 to 50 per associate) molecules (8...